Get Applications of Chlorophyll Fluorescene in Photosynthesis PDF

By G. Heinrich Krause, Engelbert Weis (auth.), Hartmut K. Lichtenthaler (eds.)

ISBN-10: 9400928238

ISBN-13: 9789400928237

ISBN-10: 9401077711

ISBN-13: 9789401077712

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Additional resources for Applications of Chlorophyll Fluorescene in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing: An introduction to the various fields of applications of the in vivo chlorophyll fluorescence also including the proceedings of the fir

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Bi! ~ i 0_ b~,: U 10 nr b i 90 J;:0i! < ~ l1. J - W PC PARTICLES 91 "'0 • PE V PC l1. :::J (/) CJ '" Z iii _ - - PARTICLES iii 0 <11 :i 0 w 10 20 P-700 CONCENTRATION Ipmo1l100 ~ll z w u PE PARTICLES (/) w a:: 0 ",- £ ~ :::J -' THYlAKOIQS "- 400 Z l1. ----- 0 iii (/) >: >::: z W :::J W -' U z THYLAKOIDS (/) w u (/) w a:: W a:: TRI TO N-TREATED THYLAKOIOS"" 200 - - - PARTICLES CJ • • 0 :::J -' l1. 600 700 SOO EMISSION WAVELENGTH Inml _ _ _ Jl 0 10 P-700 CONCENTRATION "'G PE PC 20 Ipmo1l100 ~ll Fig.

R. (1986) Biochim. Biophys. A. and Melis, A. (1988) Biochim. Biophys. R. (1950) Plant Physiol. R. W. (1962) Plant Physiol. 37,814-820 Hodges, M. and Barber, J. (1983) FEBS Lett. 160, 177-181 Hodges, M. and Barber, J. (1986) Biochim. Biophys. , Droppa, M. and Melis, A. (1984) Photobiochem. Photobiophys. , Droppa, M. and Vigh, L. (1987) Biochim. Biophys. A. A. (1977) FEBS Lett. • Haworth, P. J. (1982) Biochim. Biophys. K. and Andersson, B. (1985) Biochim. Biophys. , Oquist, G. and Andersson, B. (1986) Photobiochem.

Often the resulting amplitudes A are plotted vs. A exc or A em I which is then called a decay-associated excitation or emission spectrum (DAS), respectively (Wendler and Holzwarth 1987, Holzwarth et al. 1987). The usefulness of such plots becomes apparent from eqn. 2 which provides the relationship between the amplitude Ai in a DAS and various molecular parameters of the antenne pigments. In many cases this relationship is simply given by (2) where C is a proportionality constant, exc is the excitation intensity, € exc the absorption coefficient at the excitation wavelength \xc' NChl the size of the antenna pool (number of Chis) giving rise to decay component i, F(A ) the normalized em fluorescence spectrum of this antenna pool and kra d the radiative lifetime of the pigments.

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Applications of Chlorophyll Fluorescene in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing: An introduction to the various fields of applications of the in vivo chlorophyll fluorescence also including the proceedings of the fir by G. Heinrich Krause, Engelbert Weis (auth.), Hartmut K. Lichtenthaler (eds.)


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