By Shinji Iijima (Editor), Ken-ichi Nishijima (Editor)
Whole updates of quickly increasing fields of animal telephone know-how Covers all issues from educational to business issues
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Additional resources for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 17th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), ... Cell Technology: Basic & Applied Aspects)
However, neutralizing the antibody against TNF-α ҏalmost completely suppressed the LDH release from the Caco-2 monolayers (Fig. 3), suggesting that TNF-α ҏwas the active factor secreted from the THP-1cells that induced damage to the Caco-2 cell monolayers. It was also revealed that exposure of the Caco-2 cell monolayers to TNF-α significantly increased the LDH release (data not shown). This result strengthens the supposition that damage to the Caco2 cell monolayers had been caused by TNF-α secreted from THP-1 cells.
These results suggest that the Caco-2 cell monolayers had been damaged by coculturing the macrophage-like THP-1 cells. We next investigated whether the conditioned medium of THP-1 cells could reproduce the phenomena from the coculture with THP-1 cells. Caco-2 cell monolayers cultured in semipermeable membrane inserts were incubated with the conditioned medium prepared from THP-1 for 48 hours. As a result, the conditioned medium of THP-1 cells reproduced the damage to the Caco-2 cell monolayers (data not shown).
1994; 93 (1) Part 1: 93-99. 15. de Jong, E. , Martens, B. P. , Kapsenberg, M. , Penninks, A. , and Weirenga, E. , Allergen, IgE, mediators, Inflammatory mechanisms: Food allergen (peanut)-specific Th2 clones generated from the peripheral blood of a patient with peanut allergy. J. Allergy Clin. Immunol. 1996; 98 (1): 73-81. 16. Higgins J. , Lamb, J. , Lake, R. , and O’Hehir, R. , Polyclona and clonal + analysis of human CD4 T-lymphocyte response to nut extracts. Immunol. 1995; 84: 91-97. 17. , and Okumura, Y.
Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 17th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), ... Cell Technology: Basic & Applied Aspects) by Shinji Iijima (Editor), Ken-ichi Nishijima (Editor)