Animal Cell Culture: A Practical Approach - download pdf or read online

By John R. W. Masters

ISBN-10: 0199637962

ISBN-13: 9780199637966

This new version of Animal mobile tradition covers new or up-to-date chapters on phone authentication, serum-free tradition, apoptosis assays, FISH, genetic amendment, scale-up, stem cellphone assays, three-d tradition, tissue engineering and cytotoxicity assays. unique protocols for a wide selection of tools give you the middle of every bankruptcy, making new method simply available. all people operating in organic and scientific study, even if in academia or a advertisement association, training phone tradition will gain tremendously from this booklet.

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Gas supply is controlled by a solenoid valve and 95% air directly. Above the set point carbon dioxide will be mixed with the air, but below this point only air will be delivered to the culture. This in itself is a controlling factor in that it helps remove carbon dioxide as well as meeting the oxygen requirements of the cultures. pH regulation is very readily adapted to computer control systems. 7 Oxygen The scale-up of animal cell cultures is very dependent upon the ability to supply sufficient oxygen without causing cell damage.

41. Soule, H. , Maloney, T. , Wolman, S. , Peterson, W. , McGrath, C. , et al. (1990). , 50, 6075. 42. Giard, D. , Aaronson, S. , Todaro, G. , Kersey, J. , et al. (1973). J. Natl. , 51, 1417. 43. Greene, L A. and Tischler, A. S. (1976). Proc. Natl. Acad. Sci. USA, 73, 2424. 44. , et al. (1979). Blood, 54, 713. 45. Gillis, S. and Watson, J. (1980). J. Exp. , 152, 1709. 46. Andersson, L. , and Gahmberg, C. G. (1979). Int. J. Cancer, 23, 143. 47. Andersson, L. , and Gahmberg, C. G. (1979). Nature, 278, 364.

Medium can also deteriorate without a major pH change, as some constituents, like glutamine, are unstable, and others may be utilized without a major pH shift. It is, therefore, recommended that the medium is changed at least once per week. 5 Contamination The problem of microbial contamination has been greatly reduced by the use of laminar airflow cabinets. The risk of contamination can also be reduced by use of antibiotics, but this should be reserved for high-risk procedures, such as primary culture, and cultures should be maintained in the absence of antibiotics so that chronic, cryptic contaminations are not harboured.

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Animal Cell Culture: A Practical Approach by John R. W. Masters

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